r/proteomics • u/Triple-Tooketh • Jul 21 '26

Detergent removal

Has anyone tried this product: Pierce® Detergent Removal Spin Plates

1 Upvotes

60% Upvoted

7 comments sorted by

9

u/KillNeigh Jul 21 '26

In my experience it gets most of the detergent but not everything.

I prefer to use s-trap with samples that have detergents.

7

u/Gloomy-Gazelle-9324 Jul 21 '26

Have you tried SP3 magnetic beads? Work very well.

3

u/Discount_Sausage Jul 21 '26

S-Trap or beads. If a small set of samples, I prefer the S-Trap.

2

u/tsbatth Jul 21 '26

Magnetic beads and the PAC/SP3 method should work.

1

u/LC-MS Jul 22 '26

Hi PAC man, do you know up to what % SDS that PAC works? I haven't tried it above 1%

2

u/tsbatth Jul 22 '26

Should work up to 10% SDS ☺️. Remember to sonicate or benzonase if it’s guey and low viscosity or else that messes with the aggregation process.

0

u/flying_midget Jul 21 '26

The HiPPR never fully cleaned RIPA/SDS for me. Strap is my fave.